1191 Immunotherapy with 4-1BBL-expressing iPSC-derived proliferating myeloid cells amplifies antigen-specific T cell infiltration in advanced melanoma
نویسندگان
چکیده
Among advanced melanomas, approximately 60% of the patients present with immunologic ignorance or intrinsic induction tumors need other therapeutic approaches to change an immunologically “cold tumor” “hot by attracting T cells into tumors. We have established immune cell therapy immortalized induced pluripotent stem–cell (iPSC)–derived proliferating myeloid (iPSC–pMCs). The benefits using iPSC−pMCs are infinite proliferative capacity and ease genetic modification. In this study, we introduced 4-1BBL gene iPSC–pMCs a background C57BL6 (iPSC–pMCs–41BBL). Cytokine array analysis was performed supernatants spleen that were cocultured withiPSC–pMCs iPSC–pMCs–41BBL. Multiple cytokines beneficial cancer immunotherapy upregulated. Peritoneal injections iPSC–pMCs–41BBL inhibited tumor growth peritoneally disseminated mouse melanoma prolonged survival mice than iPSC–pMCs. Furthermore, numbers antigen-specific CXCR6+CD8 significantly increased in tissues treated epitope peptide-pulsed These results suggest iPSC−pMCs−41BBL could activate antigen–specific promote their infiltration tissues. future clinical applications, plan use human allogenic treat patients. examined anticancer effects embryonic stem (ES) cell-derived pMC from 129Sv strain, which has different MHC class II compared mouse. Allogenic ES–pMC show suggested 4-1BBL-targeted strategies also work environments.
منابع مشابه
Preferential T cell Expansion by Artificial Antigen Presenting Cells Expressing 4-1BBL Alone or in Combination with CD80 or CD86
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2023
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2023.03.1205